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The anti-bactericidal effects of FBW7 silence in macrophage(PDF)


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The anti-bactericidal effects of FBW7 silence in macrophage
Sun Jinxia1 Huang Zhong1 Wang Rui2 Weng Linjun3 and Li Yaxu3
1) Health Science Center, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2) College of Pharmacy, Liaocheng University, Liaocheng 252059, Shandong Province, P.R.China
3) School of Life Science and Technology, Tongji University, Shanghai 200092, P.R.China
small interfering RNA (siRNA) RAW264.7 macrophage bactericidal activity nitric oxide synthase (iNOS) NF-κB signaling pathway real time quantitative polymerase chain reaction (RT-qPCR)
R 392
SiRNA-mediated FBW7 silence in Hela, primary macrophage and RAW264.7 were performed to assay the role of FBW7 on NF-κB activity, cytokines expression and phagocytosis and bactericidal activity of macrophage. Flow cytometry (FCM), antibiotic protection assay (APA), real time quantitative polymerase chain reaction (RT-qPCR) and report gene analysis suggest that FBW7 silence does not affect phagocytosis of RAW264.7, but significantly inhibits bactericidal activity and nitric oxide synthase (iNOS) expression in primary macrophage and RAW264.7, suppresses NF-κB report gene activity in Hela, decreases expression of TNFα、 IL-1β、 IL-6 in Hela and MCP-1、 IL-1β in primary macrophage and RAW264.7. Thus, FBW7 silence might inhibit bactericidal activity of macrophage by regulating NF-κB activity.


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Last Update: 2017-06-26