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Characteristics of antioxidant activity of soybean PM1 protein and enhancement of tolerance of recombinant yeast to copper stress(PDF)


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Characteristics of antioxidant activity of soybean PM1 protein and enhancement of tolerance of recombinant yeast to copper stress
Li Chengna Gao Yang Liu Ziming Liu Guobao and Zheng Yizhi
College of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbiology and Gene Engineering, Shenzhen 518060, Guangdong Province, P.R.China
soybean GmPM1 protein histidine Cu2+ stress scavenging hydroxyl radicals recombinant yeast
Q 943.2;Q 71
Cu2+ is an essential micronutrient for plant growth, but it is toxic when plant growth under excess copper stress. Soybean GmPM1 protein belongs to late embryogenesis abundant (LEA) group 4 (LEA4) proteins, which has a high proportion of histidine residues in the protein sequence. Firstly, we investigate the protective function and mechanisms of GmPM1 protein in plant under Cu2+ stress. The leaves of soybean seedling are withered under 150 μmol/L CuSO4 stress, and at the meantime the expression of GmPM1 gene in the young leaves was up-regulated in 3 h and 24 h of the stress. Secondly, the yeast expression plasmid of pYES2-GmPM1 is constructed and then transformed into the copper-sensitive yeast mutant ΔYAP1 to create recombinants of ΔYAP1-GmPM1. The recombinant yeast expressing GmPM1 protein could enhance the tolerance to Cu2+ stress. Then, the activities of scavenging hydroxyl radicals of GmPM1 and GmPM1-C protein in vitro are determined by using Cu-ascorbic acid system, which is rich in histidine residual in their sequence. The results show that GmPM1 could chelate Cu2+ through histidine residual in the C-terminal of GmPM1 protein and exert the activity of scavenge hydroxyl radicals, thus could improve the tolerance of plants to Cu2+ stress.


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Last Update: 2017-09-11