|Table of Contents|

Application of DH-PSF three-dimensional dynamic multi-particle tracking method in living cell imaging(PDF)

《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

Issue:
2017年No.5(441-550)
Page:
526-531
Research Field:
光电工程
Publishing date:

Info

Title:
Application of DH-PSF three-dimensional dynamic multi-particle tracking method in living cell imaging
Author(s):
Li Heng1 2 Chen Danni1 Yu Bin1 Niu Hanben1 and Guo Baoping1
1) College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2) College of Information Engineering, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
Keywords:
optical engineering three-dimensional imaging multi-particle point spread function nano-posi-tioning living cell dynamic tracking
PACS:
Q 63;O 437
DOI:
10.3724/SP.J.1249.2017.05526
Abstract:
Multi-particle tracking is one of the most challenging aspects of living cell imaging. We establish a three-dimensional multi-particle tracking imaging system based on three-dimensional positioning of double helix point spread function.The imaging depth and localization precision can achieve 4 μm and 20 nm, respectively. The result of dynamic tracking of fluorescence microspheres in Raw cells shows that the detectable trajectory range of intracellular moleculesis could be up to 7 μm and the velocity of probes could be obtained in real time. Therefore, this system is a very useful tool for studying the intermolecular interaction in living cell.

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Last Update: 2017-09-05