[1]刘立新,王 磊,屈军乐,等.两维荧光光谱技术及最佳光谱分辨率设计[J].深圳大学学报理工版,2007,24(3):252-256.
 LIU Li-xin,WANG Lei,QU Jun-le,et al.Technique of two-dimensional fluorescence spectroscopy and design for optimal spectral resolution[J].Journal of Shenzhen University Science and Engineering,2007,24(3):252-256.
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两维荧光光谱技术及最佳光谱分辨率设计()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第24卷
期数:
2007年3期
页码:
252-256
栏目:
出版日期:
2007-07-30

文章信息/Info

Title:
Technique of two-dimensional fluorescence spectroscopy and design for optimal spectral resolution
文章编号:
1000-2618(2007)03-0252-05
作者:
刘立新12王 磊1屈军乐1林子扬1刘文清2牛憨笨1
1)深圳大学光电工程学院,深圳 518060;
2)中国科学院安徽光学精密机械研究所,合肥 230031
Author(s):
LIU Li-xin12WANG Lei1QU Jun-le1LIN Zi-yang1LIU Wen-qing2and NIU Han-ben1

1)College of Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,P.R.China
2)Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Anhui 230031,P.R.China

关键词:
荧光光谱技术光谱分辨率棱镜微透镜阵列生物医学
Keywords:
fluorescence spectroscopy technique spectral resolution prism microlens arraybiomedicine
分类号:
TH 74;O 43
文献标志码:
A
摘要:

研究和发展了一种两维荧光光谱测量及成像技术,讨论获得两维空间离散点的最佳光谱分辨率的方法.该技术以棱镜色散原理为基础,利用微透镜阵列实现对样品的离散化照明,充分发挥光学系统固有的并行处理能力,实现了在一个面阵探测器上同时记录两维空间的荧光光谱图像.与逐点扫描方法相比,该技术的扫描效率随激发点数线性提高,具有快速测量、高空间和高光谱分辨的特点,可用于生物医学领域.

Abstract:

A 2D fluorescence spectroscopy technique was proposed,and a design method for optimal spectral resolution of 2D discrete points was discussed.This new technique was based on prism dispersion theory.It emploied a microlens array for discrete illumination on the samples.With the intrinsically parallel processing capability of the optical elements and system,the 2D spectrally resolved images can be recorded with one array detector at the same time.Compared with the pixel-by-pixel laser scanning technique,the scanning efficiency of this technique is improved in linear proportion to the excitation spots.Due to its capability of quick measurement and high spatial and spectral resolutions,this technique is expected to have wide applications in biomedicine field.

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更新日期/Last Update: 2007-12-06