[1]孙锦霞,黎丽,王瑞,等.REGγ对LPS介导的腹膜炎调节作用[J].深圳大学学报理工版,2017,34(No.5(441-550)):471-475.
 Sun Jinxia,Li Li,Wang Rui,et al.Regulation of REGγ on LPS-mediated peritonitis[J].Journal of Shenzhen University Science and Engineering,2017,34(No.5(441-550)):471-475.
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REGγ对LPS介导的腹膜炎调节作用()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第34卷
期数:
2017年No.5(441-550)
页码:
471-475
栏目:
生物工程
出版日期:
2017-09-30

文章信息/Info

Title:
Regulation of REGγ on LPS-mediated peritonitis
作者:
孙锦霞1黎丽1王瑞2黄钟1
1)深圳大学医学部,广东深圳518060;2)聊城大学药学院,山东聊城252059
Author(s):
Sun Jinxia1 Li Li1 Wang Rui2 and Huang Zhong1
1)Health Science Center, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China2) College of Pharmacy, Liaocheng University, Liaocheng 252059, Shandong Province, P.R.China
关键词:
腹膜炎蛋白酶体过继转移骨髓嵌合体造血系统酶联免疫吸附测定法
Keywords:
peritonitis proteasome adoptive transfer hematopoietic system enzyme-linked immunosorbent assay
文献标志码:
A
摘要:
为研究蛋白酶体激活因子REGγ对脂多糖(lipopolysaccharides,LPS)介导的腹膜炎的调节作用,采用腹腔注射LPS的方法以建立小鼠腹膜炎模型.首先,通过骨髓细胞过继转移的方法构建骨髓嵌合体小鼠;利用存活率实验检测REGγ敲除对小鼠生存率的影响;然后,通过酶联免疫吸附测定实验检测促炎因子的表达. 结果显示,与野生型小鼠相比,REGγ敲除小鼠(KO)表现为较低的存活率,且细胞因子TNFα(tumor necrosis factor-α)、IL-1β(interleukin-1β)和MCP-1(monocyte chemoattractant protein-1)的表达也较低.提取WT和KO小鼠来源的骨髓细胞,分别过继转移至X-射线辐照过的WT小鼠,获得WTchimera和KOchimera骨髓嵌合体小鼠. 腹腔注射LPS于WTchimera和KOchimera嵌合体小鼠,结果发现,KOchimera也表现为较低的存活率和TNFα、IL-1β和MCP-1表达.研究结果表明,REGγ在造血系统来源的炎症细胞中敲除,可以加速LPS介导的小鼠存活率下降,且抑制机体促炎因子TNFα、IL-1β和MCP-1的表达.
Abstract:
Mouse peritonitis model was established by LPS peritoneal injection to analyze the roles of proteasome activator REGγ in LPS-mediated peritonitis. Bone marrow chimera mice are generated by adoptive transfer of bone marrow cells. Survival assay is used to research the effect of REGγ deficiency on survival rate. Expression of proinflammatory factors are detected via enzyme-linked immunosorbent assay (ELISA). The results indicate that REGγ-knockout (KO) mice show significantly lower survival rate as well as proinflammatory factors expression of TNFα, IL-1β and MCP-1 than wild-type (WT) mice. Furthermore, bone marrow from WT and KO mice are isolated and transferred adoptively to X-ray irradiated WT mice to generate WTchimera and KOchimera bone marrow chimera mice. The results suggest that KOchimera mice also manifest obviously lower survival rate and proinflammatory factors expression of TNFα, IL-1β and MCP-1 after peritoneal injection of LPS to WTchimera and KOchimera. Thus, these data suggests that REGγ deficiency in inflammatory cells of hematopoietic origin accelerates LPS-mediated decline of survival rate and suppresses expression of proinflammatory factors TNFα, IL-1β and MCP-1.

相似文献/References:

[1]孙锦霞,王瑞,黄钟.REGγ敲除对细胞浸润的抑制作用[J].深圳大学学报理工版,2017,34(No.2(111-220)):111.[doi:10.3724/SP.J.1249.2017.02111]
 Sun Jinxia,Wang Rui,and Huang Zhong.The inhibitory effect of REGγ-knockout on cell infiltration[J].Journal of Shenzhen University Science and Engineering,2017,34(No.5(441-550)):111.[doi:10.3724/SP.J.1249.2017.02111]

更新日期/Last Update: 2017-09-11