[1]唐玉林[],Grill Erwin[].拟南芥谷氨酸-tRNA合成酶(AtGluRS)不能取代酵母GluRS[J].深圳大学学报理工版,2006,23(3):237-241.
 TANG Yu-lin and Grill Erwin.Arabidopsis GIuRS is not able to substitute for the yeast GIuRS[J].Journal of Shenzhen University Science and Engineering,2006,23(3):237-241.
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拟南芥谷氨酸-tRNA合成酶(AtGluRS)不能取代酵母GluRS()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第23卷
期数:
2006年3期
页码:
237-241
栏目:
生命科学
出版日期:
2006-07-30

文章信息/Info

Title:
Arabidopsis GIuRS is not able to substitute for the yeast GIuRS
文章编号:
1000-2618(2006)03-0237-05
作者:
唐玉林[1] Grill Erwin[2]
1) 深圳大学生命科学学院,深圳5180602)慕尼黑工业大学植物所,福莱津85354,德国
Author(s):
TANG Yu-lin1 and Grill Erwin2

1)College of Life Science Shenzhen University
2)Lehrstuhl fuer Botanik Technische Universitaet Muenchen

关键词:
谷氨酸-tRNA合成酶(GluRS)基因功能互补拟南芥酵母
Keywords:
Glutamyl-tRNA synthetase functional complementation Arabidopisis yeast
分类号:
Q 934.2; Q 786
文献标志码:
A
摘要:
将拟南芥细胞质谷氨酸-tRNA合成酶(AtGluRS)引入酵母细胞,研究其与酵母GluRS进行功能互补的可能性.无功能的酵母GluRS会导致酵母细胞死亡,将携带AtGluRS的pACT2-AtGluRS或pBridge(dNLS)-AtGluRS表达载体转化到含有能表达酵母谷氨酸-tRNA合成酶的pRS316-yGluRS载体的gluRS缺陷型酵母菌株中,若拟南芥GluRS能取代酵母中的该同源蛋白,酵母细胞中的载体pRS316-yGluRS在非选择性培养条件下将会很快丢失.然而,在经过3d的非选择性培养后,发现酵母细胞没有因拥有AtGluRS而失去提供GluRS功能的载体pRS316-yGluRS.可见,不同物种的GluRS之间,即使具有很高的同源性,它们在演化过程中可能形成了各自不同的特性,导致了拟南芥AtGluRS不能对酵母GluRS起功能互补作用.提示一些同源蛋白在异源生物体中不能发挥其正常功能.
Abstract:
The cytosolic glutamy-tRNA synthetase gene(GluRS) of Arabidopisis was introduced into yeast to test for functional complementation of the yeast homologue. Function loss of the yeast GluRS is lethal. A mutant of Saccharomyces carrying the yeast GluRS gene on plasmid pRS316 was co-transfected with a second plasmid expressing Arabidopisis GluRS. Provided that the plant GluRS can substitute for the yeast homologue, the pRS316 will be efficiently lost without selection for the plasmid. Indeed, yeasts were cured of pRS316 with high efficiency in case where the yeast GluRS gene was present elsewhere. However, the Arabidopsis GluRS was not able to substitute for the yeast homologue and no yeasts were observed that lost pRS316 as the only source of yeast GluRS function. The results indicate that the GluRS, though encoding an evolutionary well-conserved protein,has evolved functional specialties that prevent the complementation of yeast by the plant GluRS. It presents an example for the fact that not all the homology proteins function well in a diverse foreign organism.
更新日期/Last Update: 2015-06-26