[1]胡章立,舒龙飞,苟德明.莱茵衣藻硫胁迫相关microRNA检测及其靶基因分析[J].深圳大学学报理工版,2011,28(No.3(189-282)):237-242.
 HU Zhang-li,SHU Long-fei,and GOU De-ming.MicroRNAs quantification and related target genes for response to sulfur deprivation in Chlamydomonas reinhardtii[J].Journal of Shenzhen University Science and Engineering,2011,28(No.3(189-282)):237-242.
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莱茵衣藻硫胁迫相关microRNA检测及其靶基因分析()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第28卷
期数:
2011年No.3(189-282)
页码:
237-242
栏目:
生物工程
出版日期:
2011-05-20

文章信息/Info

Title:
MicroRNAs quantification and related target genes for response to sulfur deprivation in Chlamydomonas reinhardtii
文章编号:
1000-2618(2011)03-0237-06
作者:
胡章立舒龙飞苟德明
深圳市海洋生物资源与生态环境重点实验室,深圳大学生命科学学院,深圳 518060
Author(s):
HU Zhang-liSHU Long-feiand GOU De-ming
Shenzhen Key Laboratory for Marine Bioresource and Eco-environmental Science, College of Life Sciences, Shenzhen University, Shenzhen 518060, P.R.China
关键词:
生物工程分子生物学莱茵衣藻缺硫胁迫小分子核糖核酸实时定量反转录聚合酶链式反应
Keywords:
bioengineeringmolecular biologyChlamydomonas reinhardtiisulfur deprivationmicro ribonucleic acid(microRNA)real-time RT-PCR
分类号:
Q 74
文献标志码:
A
摘要:
采用特异反转录引物,构建莱茵衣藻microRNA(miRNA)的cDNA文库.选用U4核仁小分子RNA(small nucleolar RNA,snoRNA)作为内参,用SYBR green RT-PCR对3种与莱茵衣藻缺硫胁迫反应相关的miRNA进行检测,利用在线平台软件Web MicroRNAs Designer(WMD3) 对miRNA靶基因进行预测.结果表明,在缺硫胁迫下,3种miRNA(miRNA1145.2、miRNA1146和 miRNA1158)的表达水平均明显上调,与正常培养的莱茵衣藻表达miRNA的相对丰度比值分别为3.11、2.38和3.67.靶基因预测分析表明,miRNA1145.2能影响脂肪酸氧化反应,miRNA1146与辅酶Q代谢相关,miRNA1158可能参与磷酸戊糖途径调控.
Abstract:
Two small RNA libraries,which responded to sulfur-replete and sulfur-deprivation condition,were generated by using stem-loop primers in Chlamydomonas reinhardtii. Three microRNAs (miRNA1145.2,miRNA1146 and miRNA1158) were quantified by using SYBR green reverse transcription polymerase chain reaction(RT-PCR) detection with U4 snoRNA as internal reference gene.Their target genes were predicted by the Web MicroRNAs Designer(WMD3).The results showed that all three microRNAs expressions were up-regulated under sulfur deprivation in Chlamydomonas reinhardtii. The ratio of relative abundance with/without sulfur deprivation for miRNA1145.2,miRNA1146 and miRNA1158 were 3.11,2.38 and 3.67,respectively.The miRNA1145.2 was able to target the thiolase gene to influence the fatty acid metabolism.The miRNA1146 targeted the genes for ubiquinone/menaquinone biosynthesis methyltransferase to control the ubiquinone metabolism.The miRNA1158 targeted the 6-phosphogluconate dehydrogenase genes to regulate the pentose phosphate pathway. Results show that the photosynthetic metabolism could be changed by the expression of endogenous miRNAs to regulate their target genes. And we can also design miRNAs to regulate any specific genes that catch our attention.

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备注/Memo

备注/Memo:
收稿日期:2010-07-12;修回日期:2010-11-19
基金项目:国家自然科学基金资助项目(31070323)
作者简介:胡章立(1964-),男(汉族),湖南省澧县人,深圳大学教授、博士生导师.E-mail:huzl@szu.edu.cn
更新日期/Last Update: 2011-05-23