[1]倪卓,王应,王双,等.PEEK-HA生物复合材料对MG-63细胞的作用[J].深圳大学学报理工版,2014,31(No.3(221-330)):258-265.[doi:10.3724/SP.J.1249.2014.03258]
 Ni Zhuo,Wang Ying,Wang Shuang,et al.Influence of PEEK-HA composites on MG-63 cells[J].Journal of Shenzhen University Science and Engineering,2014,31(No.3(221-330)):258-265.[doi:10.3724/SP.J.1249.2014.03258]
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PEEK-HA生物复合材料对MG-63细胞的作用()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第31卷
期数:
2014年No.3(221-330)
页码:
258-265
栏目:
化学与化工
出版日期:
2014-05-20

文章信息/Info

Title:
Influence of PEEK-HA composites on MG-63 cells
文章编号:
201403007
作者:
倪卓1王应2王双1刘士德2张建华2刘学3任英华3
1) 深圳大学化学与化工学院,深圳 518060
2) 深圳大学生命科学学院,深圳 518060
3) 深圳市第六人民医院,深圳 518067
Author(s):
Ni Zhuo1 Wang Ying2 Wang Shuang1 Liu Shide2 Zhang Jianhua2 Liu Xue3 and Ren Yinghua3
1) College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, P.R.China
2) College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
3) Shenzhen Sixth People’s Hospital, Shenzhen 518067, P.R.China
关键词:
生物材料学复合材料骨替代材料聚醚醚酮-磺化聚醚醚酮-羟基磷灰石复合材料成骨MG-63细胞黏附率细胞增殖细胞凋亡
Keywords:
biomaterials science composites bone substitute materials polyetheretherketone-sulfnated polyetheretherketone-hydroxyapatite(PEEK-SPEEK-HA)composites MG-63 cells cell adhesion proliferation apoptosis
分类号:
TB 332;R 318.08
DOI:
10.3724/SP.J.1249.2014.03258
文献标志码:
A
摘要:
体外培养成骨MG-63细胞, 通过倒置荧光显微镜和四甲基偶氮唑盐微量酶反应比色法,研究聚醚醚酮-磺化聚醚醚酮-羟基磷灰石(polyetheretherketone-sulfnated polyetheretherketone-hydroxyapatite, PEEK-SPEEK-HA)复合材料对细胞形态和增殖的影响.在PEEK-SPEEK-HA复合材料质量浓度为4 mg/mL时,复合材料对MG-63细胞增殖作用最强,随浸提液质量浓度增大,材料对细胞增殖作用下降.材料质量浓度为4 mg/mL时,HA质量分数为20%的PEEK-SPEEK-HA复合材料对细胞的增殖作用较强. PEEK-HA和PEEK-SPEEK-HA复合材料的毒性评级为1级,无细胞毒性. SPEEK和HA能够改善复合材料对成骨MG-63细胞的黏附作用,HA含量对细胞黏附作用的影响更显著. 扫描电镜结果表明MG-63细胞在PEEK-SPEEK-HA材料上生长形态为三角形或长梭形,有伪足,伸展状态良好,细胞可以在材料缝隙处生长,表明成骨细胞能够在该材料表面黏附、伸展和生长. 将SPEEK引入PEEK-HA复合材料能抑制细胞凋亡.
Abstract:
Toxicity effects of PEEK-SPEEK-HA composite materials on the MG-63 cells are studied in this paper. MTT results show that when the concentration of extracts increases, the cell proliferation decreases. In a concentration of 4 mg/mL,this material plays a significant role in cell proliferation. A concentration of 4 mg/mL has been selected to study the cell proliferation effect of different materials, and it is found that PEEK-SPEEK-HA(w(HA)=20%) composites have a stronger effect on cell proliferation than that of PEEK-HA(w(HA)=20%).The cytotoxicity of PEEK-SPEEK-HA and PEEK-HA composite is level 1, indicating no cytotoxicity. The cell adhesion rate increases with the increase of HA content in PEEK-HA composites, and scanning electron microscope (SEM) images show that MG-63 cells can adhere, spread and grow on PEEK-SPEEK-HA surfaces. Apoptosis experiments show that PEEK-SPEEK-HA composite materials can inhibit apoptosis.

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备注/Memo

备注/Memo:
Received:2014-02-25;Accepted:2014-04-28
Foundation:Science and Technology Innovation Project of Guangdong Provincial Educational Department(2013KJCX0163);Applied Technology Development Project of Shenzhen University (201223)
Corresponding author:Professor Ni Zhuo. E-mail:royzhuoni@hotmail.com
Citation:Ni Zhuo,Wang Ying,Wang Shuang,et al. Influence of PEEK-HA composites on MG-63 cells[J]. Journal of Shenzhen University Science and Engineering, 2014, 31(3): 258-265.(in Chinese)
基金项目:广东省教育厅科技创新项目(2013KJCX0163);深圳大学应用技术开发项目(201223)
作者简介:倪卓(1963—),男(汉族),吉林省通化市人,深圳大学教授、博士生导师. E-mail:royzhuoni@hotmail.com
引文:倪卓,王双,王应,等. PEEK-HA生物复合材料对MG-63细胞的作用[J]. 深圳大学学报理工版,2014,31(3):258-265.
更新日期/Last Update: 2014-05-04