[1]孙锦霞,王瑞,黄钟.REGγ敲除对细胞浸润的抑制作用[J].深圳大学学报理工版,2017,34(No.2(111-220)):111-116.[doi:10.3724/SP.J.1249.2017.02111]
 Sun Jinxia,Wang Rui,and Huang Zhong.The inhibitory effect of REGγ-knockout on cell infiltration[J].Journal of Shenzhen University Science and Engineering,2017,34(No.2(111-220)):111-116.[doi:10.3724/SP.J.1249.2017.02111]
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REGγ敲除对细胞浸润的抑制作用()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第34卷
期数:
2017年No.2(111-220)
页码:
111-116
栏目:
生物工程
出版日期:
2017-03-20

文章信息/Info

Title:
The inhibitory effect of REGγ-knockout on cell infiltration
文章编号:
201702001
作者:
孙锦霞1王瑞2黄钟1
1)深圳大学医学部,广东深圳518060
2)聊城大学药学院,山东聊城252059
Author(s):
Sun Jinxia1 Wang Rui2 and Huang Zhong1
1) Health Science Center, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2) College of Pharmacy, Liaocheng University, Liaocheng 252059, Shandong Province, P.R.China
关键词:
败血症蛋白酶体李斯特菌中性粒细胞单核细胞细胞浸润克隆形成实验流式细胞术苏木精-伊红染色
Keywords:
sepsis proteasome Listeria Monocytogenes neutrophil monocyte cell infiltration clone formation assay flow cytometry hematoxylin-eosin staining
分类号:
R 392
DOI:
10.3724/SP.J.1249.2017.02111
文献标志码:
A
摘要:
研究蛋白酶体激活因子REGγ对免疫细胞浸润能力的影响,分别腹腔注射李斯特菌于野生型和REGγ敲除小鼠,通过存活率检测、克隆形成、流式细胞术和苏木精-伊红染色实验研究,发现REGγ敲除小鼠表现为较低的存活率和李斯特菌清除能力.REGγ敲除显著抑制了中性粒细胞和单核细胞向感染部位的浸润.表明REGγ敲除后免疫细胞不能募集至感染部位,导致细菌未能被有效清除而在体内大量繁殖,最终引发败血症的发生,降低小鼠的存活率.
Abstract:
Peritoneal injections of Listeria Monocytogenes to wild-type and REGγ-knockout mice were performed for analyzing the roles of proteasome activator REGγ in immune cell infiltration. By survival assay, clone formation assay, flow cytometry and hematoxylin-eosin staining, we found that mice lacking REGγ shows significantly lower survival and Listeria Monocytogenes clearance. Furthermore, REGγ deficiency obviously inhibits the infiltration of neutrophil and monocyte to infection sites. Thus, the results suggest that REGγ deficiency suppresses immune cell recruitment to infection sites,resulting in impaired bacterial clearance. Rapid bacterial reproduction causes sepsis eventually, and leads to the reduced survival rate of REGγ-knockout mice.

参考文献/References:

[1] Scallan E, Hoekstra R M, Angulo F J, et al. Foodborne illness acquired in the United States-major pathogens[J]. Emerging Infectious Diseases, 2011, 17(1): 7-15.
[2] Ribet D, Cossart P. How bacterial pathogens colonize their hosts and invade deeper tissues[J]. Microbes and Infection,2015,17(3):173-183.
[3] Khan S, Badovinac V. Listeria monocytogenes: a model pathogen to study antigen-specific memory CD8 T cell responses[J]. Seminars in Immunopathology, 2015, 37(3):301-310.
[4] Czuprynski C, Henson P, Campbell P. Killing of listeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and nonimmune mice[J]. Journal of Leukocyte Biology, 1984, 35(2): 193-208.
[5] Mao I, Liu J, Li Xiaotao, et al. REGγ, a proteasome activator and beyond?[J]. Cellular and Molecular Life Sciences, 2008, 65(24): 3971-3980.
[6] Li Xiaotao, Lonard D, Jung S, et al. The SRC-3/AIB1 coactivator is degraded in a ubiquitin- and ATP-independent manner by the REGgamma proteasome[J]. Cell,2006,124(2):381-392.
[7] Hao Ying, Furuya F, Li Zhao, et al. Aberrant accumulation of PTTG1 induced by a mutated thyroid hormone beta receptor inhibits mitotic progression[J]. The Journal of Clinical Investigation,2006,116(11):2972-2984.
[8] Zhang Zhuo, Zhang Ruiwen. Proteasome activator PA28γ regulates p53 by enhancing its MDM2-mediated degradation[J].The EMBO Journal,2008,27(6):852-864.
[9] Sun Jinxia, Luan Yi, Xiang Dong, et al. The 11S proteasome subunit PSME3 is a positive feedforward regulator of NF-κB and important for host defense against bacterial pathogens[J].Cell Reports,2016,14(4):737-749.
[10] Fang Haoshu, Jiang Wei, Cheng Jin, et al. Balancing innate immunity and inflammatory state via modulation of neutrophil function: a novel strategy to fight sepsis[J]. Journal of Immunology Research, 2015, 2015(9): 187048.
[11] Ma A C, Kubes P P. Neutrophils,and neutrophil extracellular traps (NETs) in sepsis[J]. Journal of Thrombosis and Haemostasis, 2008, 6(3): 415-420.
[12] Brown K, Brain S, Pearson J, et al. Neutrophils in development of multiple organ failure in sepsis[J]. Lancet, 2006, 368(9530):157-169.
[13] Gahring L C, Osborne A V, Reed M, et al. Neuronal nicotinic alpha7 receptors modulate early neutrophil infiltration to sites of skin inflammation[J]. Journal of Neuroinflammation,2010,7(1):38.
[14] Sun Yuansheng, Sijts A J A M, Song Mingxia, et al. Expression of the proteasome activator PA28 rescues the presentation of a cytotoxic T lymphocyte epitope on melanoma cells[J]. Cancer Research, 2002, 62(10): 2875-2882.
[15] Dong Shuxian, Jia Caifeng, Zhang Shengping, et al. The REGγ proteasome regulates hepatic lipid metabolism through inhibition of autophagy[J]. Cell Metabolism, 2013,18(3):380-391.
[16] Sun Lianhui, Fan Guangjian, Shan Peipei, et al. Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome[J]. Nature Communications,2016,7: 12497.
[17] Li Shuangxi, Jiang Cong, Pan Jingjing, et al. Regulation of c-Myc protein stability by proteasome activator REGγ[J]. Cell Death Differentiation, 2015, 22(6): 1000-1011.
[18] Xu Jinjin, Zhou Lei, Ji Lei, et al. The REGγ-proteasome forms a regulatory circuit with IκBε and NFκB in experimental colitis[J]. Nature Communications, 2016,7: 10761.

相似文献/References:

[1]孙锦霞,黎丽,王瑞,等.REGγ对LPS介导的腹膜炎调节作用[J].深圳大学学报理工版,2017,34(No.5(441-550)):471.[doi:10.3724/SP.J.1249.2017.05471]
 Sun Jinxia,Li Li,Wang Rui,et al.Regulation of REGγ on LPS-mediated peritonitis[J].Journal of Shenzhen University Science and Engineering,2017,34(No.2(111-220)):471.[doi:10.3724/SP.J.1249.2017.05471]

备注/Memo

备注/Memo:
Received:2016-12-16;Accepted:2017-01-27
Foundation:National Natural Science Foundation of China (31401217); Postdoctral Science Foundation of China (2014M0560672)
Corresponding author:Professor Huang Zhong. E-mail: zhuang809@126.com
Citation:Sun Jinxia, Wang Rui, Huang Zhong. The inhibitory effect of REGγ-knockout on cell infiltration[J]. Journal of Shenzhen University Science and Engineering, 2017, 34(2): 111-116.(in Chinese)
基金项目:国家自然科学基金资助项目(31401217);中国博士后科学基金资助项目(2014M0560672)
作者简介:孙锦霞(1986—),女,深圳大学博士后研究人员. 研究方向:免疫学. E-mail:jinxia8608@126.com
引文:孙锦霞,王瑞,黄钟. REGγ敲除对细胞浸润的抑制作用[J]. 深圳大学学报理工版,2017,34(2):111-116.
更新日期/Last Update: 2017-03-03