[1]李承娜,高阳,刘子明,等.大豆PM1蛋白抗氧化作用及提高酵母对铜耐受力[J].深圳大学学报理工版,2017,34(No.5(441-550)):457-463.
 Li Chengna,Gao Yang,Liu Ziming,et al.Effects of soybean PM1 protein on antioxidative stress and increasing tolerance of recombinant yeast to copper stress[J].Journal of Shenzhen University Science and Engineering,2017,34(No.5(441-550)):457-463.
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大豆PM1蛋白抗氧化作用及提高酵母对铜耐受力()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第34卷
期数:
2017年No.5(441-550)
页码:
457-463
栏目:
生物工程
出版日期:
2017-09-30

文章信息/Info

Title:
Effects of soybean PM1 protein on antioxidative stress and increasing tolerance of recombinant yeast to copper stress
作者:
李承娜高阳刘子明刘国宝郑易之
深圳大学生命与海洋科学学院,深圳市微生物工程重点实验室,广东深圳518060
Author(s):
Li Chengna Gao Yang Liu Ziming Liu Guobao and Zheng Yizhi
College of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbiology and Gene Engineering, Shenzhen 518060, Guangdong Province, P.R.China
关键词:
大豆GmPM1蛋白组氨酸Cu2+胁迫清除羟基自由基酵母重组子
Keywords:
soybean GmPM1 protein histidine Cu2+ stress scavenging hydroxyl radicals recombinant yeast
文献标志码:
A
摘要:
Cu2+是植物生长必需的微量元素之一,但土壤中过量的Cu2+会对植物细胞产生毒害作用.大豆GmPM1蛋白属于第4组的胚胎晚期富集(late embroygenesis abundant, LEA)蛋白,其序列中富含组氨酸残基. 研究大豆GmPM1蛋白在提高植物耐Cu2+胁迫方面的保护作用及其机理. 对大豆幼苗进行150 μmol/L CuSO4胁迫实验. 结果表明,CuSO4胁迫会造成大豆幼苗叶片失水萎蔫;在胁迫3 h和24 h时,幼叶内GmPM1基因表达上调. 构建了酵母表达载体pYES2-GmPM1,转化Cu2+敏感型酵母(ΔYAP1)得到重组菌ΔYAP1-GmPM1. 检测结果表明,表达GmPM1蛋白的酵母重组子对Cu2+胁迫耐受力得到提高. 采用Cu抗坏血酸体系,在体外检测出GmPM1及富含组氨酸残基的GmPM1-C端蛋白具有清除羟基自由基能力. 研究表明,大豆GmPM1蛋白可通过其C端的组氨酸残基结合过多的Cu2+,清除由Cu2+胁迫造成的细胞内产生的过量羟基自由基,提高植物及其细胞对Cu2+胁迫的耐受性.
Abstract:
Cu2+ is an essential micronutrient for plant growth, but it is toxic when plant growth under excess copper stress. Soybean GmPM1 protein belongs to late embryogenesis abundant (LEA) group 4 (LEA4) proteins, and have a high proportion of histidine residues in the protein sequence. In this paper, we studied the protective functions and mechanisms of GmPM1 protein under Cu2+ stress. The leaves of soybean seedling were withered under 150 μmol/L CuSO4 stress, and at the meantime the expression of GmPM1 gene in the young leaves was up-regulated in 3 h and 24 h of the stress. The yeast expression plasmid of pYES2-GmPM1 was constructed and then transformed into the copper-sensitive yeast mutant ΔYAP1 to create recombinants of ΔYAP1-GmPM1. The recombinant yeast expressing GmPM1 protein could enhance the tolerance to Cu2+ stress. By using Cu-ascorbic acid system, activities of scavenging hydroxyl radicals of GmPM1 and PM1-C protein in vitro was determined, which is rich in histidine residual in their sequence. Therefore, it is suggested that GmPM1 could chelate Cu2+ through histidine residual in the C-terminal of PM1 protein and exert the activity of scavenge hydroxyl radicals, thus could improve the tolerance of plants to Cu2+ stress.

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 LIU Yun,LI Ran-hui,ZHENG Yi-zhi,et al.Soybean PM2 protein and its 22-mer region enhance salt tolerance of tobacco plants[J].Journal of Shenzhen University Science and Engineering,2007,24(No.5(441-550)):95.
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[4]余玉雯,孙海丹,郑易之,等.大豆耐盐相关基因的分离及其功能鉴定[J].深圳大学学报理工版,2004,21(4):324.
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更新日期/Last Update: 2017-09-11