[1]李恒,陈丹妮,于斌,等.DH-PSF三维动态多粒子追踪在活细胞成像的应用[J].深圳大学学报理工版,2017,34(No.5(441-550)):526-531.[doi:10.3724/SP.J.1249.2017.05526]
 Li Heng,Chen Danni,et al.Application of DH-PSF three-dimensional dynamic multi-particle tracking method in living cell imaging[J].Journal of Shenzhen University Science and Engineering,2017,34(No.5(441-550)):526-531.[doi:10.3724/SP.J.1249.2017.05526]
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DH-PSF三维动态多粒子追踪在活细胞成像的应用()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第34卷
期数:
2017年No.5(441-550)
页码:
526-531
栏目:
光电工程
出版日期:
2017-09-20

文章信息/Info

Title:
Application of DH-PSF three-dimensional dynamic multi-particle tracking method in living cell imaging
文章编号:
201705014
作者:
李恒12陈丹妮1于斌1牛憨笨1郭宝平1
1)深圳大学光电工程学院,光电子器件与系统教育部/广东省重点实验室,广东深圳 518060
2)深圳大学信息工程学院,广东深圳 518060
Author(s):
Li Heng1 2 Chen Danni1 Yu Bin1 Niu Hanben1 and Guo Baoping1
1) College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2) College of Information Engineering, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
关键词:
光学工程三维成像多粒子点扩展函数纳米定位活细胞动态追踪
Keywords:
optical engineering three-dimensional imaging multi-particle point spread function nano-posi-tioning living cell dynamic tracking
分类号:
Q 63;O 437
DOI:
10.3724/SP.J.1249.2017.05526
文献标志码:
A
摘要:
利用双螺旋点扩散函数三维定位的能力建立了三维多粒子追踪成像系统,成像深度可达到4 μm,定位精度达到20 nm,并在该系统上实现了对巨噬细胞内多个荧光微球的动态追踪.结果显示,细胞内荧光探针的运动范围达到7 μm,可实时获取荧光微球在细胞内的运动速率,研究表明该系统是生物学家研究细胞内分子转运和相互关系非常有用的工具.
Abstract:
Multi-particle tracking is one of the most challenging aspects of living cell imaging. We establish a three-dimensional multi-particle tracking imaging system based on three-dimensional positioning of double helix point spread function.The imaging depth and localization precision can achieve 4 μm and 20 nm, respectively. The result of dynamic tracking of fluorescence microspheres in Raw cells shows that the detectable trajectory range of intracellular moleculesis could be up to 7 μm and the velocity of probes could be obtained in real time. Therefore, this system is a very useful tool for studying the intermolecular interaction in living cell.

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备注/Memo

备注/Memo:
Received:2017-04-07;Accepted:2017-05-27
Foundation:National Natural Science Foundation of China ( 61605127); Natural Science Foundation of Guangdong Province (2014A030312008); Shenzhen Science and Technology Research Foundation (JCYJ20150324141711698, JCYJ20160308104404452)
Corresponding author:Associate professor Chen Danni.E-mail: dannyc007@163.com
Citation:Li Heng, Chen Danni, Yu Bin, et al.Application of DH-PSF three-dimensional dynamic multi-particles tracking method in living cell imaging[J]. Journal of Shenzhen University Science and Engineering, 2017, 34(5): 526-531.(in Chinese)
基金项目:国家自然科学基金资助项目(61605127);广东省自然科学基金资助项目(2014A030312008);深圳市科技计划资助项目(JCYJ20150324141711698, JCYJ2016030810 4404452)
作者简介:李恒(1985—),男,深圳大学博士后研究人员.研究方向:超分辨成像.E-mail:liheng@email.szu.edu.cn
引文:李恒,陈丹妮,于斌,等.DH-PSF三维动态多分子追踪在活细胞成像的应用[J]. 深圳大学学报理工版,2017,34(5):526-531.
更新日期/Last Update: 2017-09-05