[1]郭志凯,盖翠娟,蔡彩虹,等.土壤链霉菌Streptomyces sp.的抑菌成分[J].深圳大学学报理工版,2018,35(No.2(111-220)):166-171.[doi:10.3724/SP.J.1249.2018.02166]
 GUO Zhikai,GAI Cuijuan,CAI Caihong,et al.Antibacterial metabolites from the soil-derived actinobacteria Streptomyces sp.[J].Journal of Shenzhen University Science and Engineering,2018,35(No.2(111-220)):166-171.[doi:10.3724/SP.J.1249.2018.02166]
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土壤链霉菌Streptomyces sp.的抑菌成分()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第35卷
期数:
2018年No.2(111-220)
页码:
166-171
栏目:
生物工程
出版日期:
2018-03-20

文章信息/Info

Title:
Antibacterial metabolites from the soil-derived actinobacteria Streptomyces sp.
文章编号:
201802009
作者:
郭志凯盖翠娟蔡彩虹陈亮亮戴好富梅文莉
中国热带农业科学院热带生物技术研究所;农业部热带作物生物学与遗传资源利用重点实验室,海南海口 571101
Author(s):
GUO Zhikai GAI Cuijuan CAI Caihong CHEN Liangliang DAI Haofu and MEI Wenli
Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, P.R.China
关键词:
天然产物放线菌链霉菌柱层析活性代谢产物抗菌活性
Keywords:
bioactive natural products actinobacteria Streptomyces sp. secondary metabolites antibacterial activity
分类号:
Q 939.92;R 978.1
DOI:
10.3724/SP.J.1249.2018.02166
文献标志码:
A
摘要:
为从放线菌次生代谢产物中寻找结构新颖的活性先导分子,通过抗菌活性筛选,发现一株土壤链霉菌Streptomyces sp.的发酵提取物具有抑菌活性. 对该菌进行大规模发酵,利用正相、反相硅胶柱层析和半制备高效液相色谱法,从其发酵产物中分离得到化合物1—11,利用高分辨质谱、一维和二维核磁共振技术,分别将其结构鉴定为(E)-3-甲硫基丙烯酸(1)、(E)-3-甲硫基丙烯酰胺(2)、反式肉桂酸(3)、(E)-4-苯基-3-丁烯酸(4)、 3-羟基-4-甲氧基肉桂酰胺(5)、 3-indoleketol(6)、 N-乙酰基-β-氧色胺(7)、 2-乙酰基大黄素(8)、7-羟基-2-(2-羟丙基)-5-甲基色酮(9)、环(苯丙-脯)(10)和环(3-羟基酪-脯)(11). 其中,化合物4—6、8、9和11是首次从链霉菌中发现,并且首次报道了化合物2的 13C核磁数据. 体外对化合物2—4、6、8和9进行了抗菌活性测试,结果表明,化合物3和6对枯草芽孢杆菌和耻垢分支杆菌具有弱抑制活性,化合物8对枯草芽孢杆菌、金黄色葡萄球菌和耻垢分支杆菌也具有弱抑菌活性.
Abstract:
Aiming to find bioactive natural products with novel structures from the secondary metabolites of actinomycetes and through screening the in vitro antibacterial activity, we find that the fermentation broth of the soil-derived Streptomyces sp. shows inhibitory activity against several bacteria. Then the strain is fermented in large scale. On the basis of HR-ESI-MS, 1D and 2D NMR analysis, and by comparison of their NMR data with those published previously, we isolate eleven compounds (1-11) from the ethyl acetate extract using various column chromatography. Their structures are (E)-3-( methylthio)acrylic acid (1), (E)-3-(methylthio)acrylamide(2), (E)-cinnamic acid (3), (E)-4-phenyl-3-butenoic acid (4), 3-hydroxy-4-methoxycinnamamide (5), 3-indoleketol (6), N-acetyl-β- oxotryptamine (7), 2-acetylemodin (8), 7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone (9), cyclo ( Phe-Pro) (10), and (3S-cis)-hexahydro-3-[(3,4-dihydroxyphenyl)methyl]pyrrolo[1,2-a]pyrazine-1,4-dione (11) Compounds 4-6, 8, 9 and 11 are firstly discovered from Streptomyces and the 13C NMR data of compound 2 is also firstly reported, to our knowledge. Compounds 2-4, 6, 8 and 9 are assessed for in vitro antibacterial activity, with compound 3 and 6 displaying weak activity against the gram-positive Bacillus subtilis and Mycobacterium smegmatis, while compound 8 exhibiting weak activity against Bacillus subtilis and Mycobacterium smegmatis and Staphylococcus aureus
更新日期/Last Update: 2018-03-07