[1]罗秋兰,王潮岗,胡章立.莱茵衣藻APC/C复合物基因家族及CrCDC20表达谱分析[J].深圳大学学报理工版,2018,35(No.2(111-220)):172-178.[doi:10.3724/SP.J.1249.2018.02172]
 LUO Qiulan,WANG Chaogang,and HU Zhangli.Bioinformatical analysis of genes encoding APC/C and the expression profiles analysis of CrCDC20 in Chlamydomanos reinhardtii[J].Journal of Shenzhen University Science and Engineering,2018,35(No.2(111-220)):172-178.[doi:10.3724/SP.J.1249.2018.02172]
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莱茵衣藻APC/C复合物基因家族及CrCDC20表达谱分析()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第35卷
期数:
2018年No.2(111-220)
页码:
172-178
栏目:
生物工程
出版日期:
2018-03-20

文章信息/Info

Title:
Bioinformatical analysis of genes encoding APC/C and the expression profiles analysis of CrCDC20 in Chlamydomanos reinhardtii
文章编号:
201802010
作者:
罗秋兰1234王潮岗13胡章立13
1) 深圳大学生命与海洋科学学院,深圳市海洋生物资源与生态环境重点实验室,广东深圳 518060;2) 深圳大学光电工程学院,光电子器件与系统教育部/广东省重点实验室,广东深圳 518060;3) 深圳大学龙华生物产业创新研究院,深圳市海洋藻类生物工程技术研究中心,广东深圳 518060;4) 中国热带农业科学院,热带生物技术研究所,海南海口 570100
Author(s):
LUO Qiulan1234 WANG Chaogang13 and HU Zhangli13
关键词:
微藻分子生物学生物柴油细胞周期E3泛素连接酶进化树分析实时荧光定量反转录聚合酶链式反应
Keywords:
molecular biology of microalgae biodiesel cell cycle E3 ubiquitin ligase phylogenetic analysis real-time fluorescence quantitative reverse transcription polymerase chain reaction ( rt-RT-qPCR)
分类号:
Q 946.1
DOI:
10.3724/SP.J.1249.2018.02172
文献标志码:
A
摘要:
为揭示莱茵衣藻细胞周期调控机制,提高微藻生物量,通过生物信息学方法,对莱茵衣藻参与细胞周期控制的末期促进复合物(anaphase-promoting complex/cyclosome,APC/C)基因家族的性质进行系统分析,包括基因结构、蛋白质特性和进化树分析等. 结果显示,莱茵衣藻包含13个APC/C复合物编码基因,在13号染色体存在一个基因簇. 除了激活因子细胞分裂周期蛋白20(cell division cycle 20,CDC20)和CDC20同系蛋白1(CDC20 homolog 1,CDH1)相对保守外,莱茵衣藻APC/C复合物的其他亚基与高等植物来源的蛋白质保守性很低. 此外,利用实时荧光定量反转录聚合酶链式反应(real-time fluorescence quantitative reverse transcription polymerase chain reaction, rt-RT-qPCR)分析了莱茵衣藻CrCDC20基因在缺氮或缺硫下表达谱,发现CrCDC20基因在缺硫下上调表达,在缺氮下下调表达,说明CrCDC20参与莱茵衣藻的营养缺乏胁迫应答反应
Abstract:
Revealing regulation mechanisms of cell cycle can prove a new direction for promoting biomass in C. reinhardtii. In this paper, the gene structures, protein characteristics, and phylogenetic tree of anaphase-promoting complex/ cyclosome (APC/C) gene family which involving in cell cycle controlling were analyzed through bioinformatics. Totally 13 genes of APC/C family were identified in C. reinhardtii, and one gene cluster was found in chromosome 13. The phylogenetic tree showed that except activation factors CrCDC20 and CrCDH1, APC/C proteins from C. reinhardtii has low homologous with that in higher plants. Meanwhile, the gene expression profiles of CrCDC20 under sulfur or nitrogen deficiency conditions were determined by real-time fluorescence quantitative reverse transcription polymerase chain reaction ( rt-RT-qPCR). The results showed that CrCDC20 up-regulate under sulfur deficiency but down-regulate under nitrogen deficiency, which indicates that CrCDC20 might involve in nutrition deficiency stresses
更新日期/Last Update: 2018-03-07